Virus:Article Title: Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal Anti-Bassoon, 1:800 Enzo CAT# MA1-20689; RRID: AB_2066981 Mouse monoclonal Anti-ARR3, 1:500 Gift from the Laboratory of Wolfgang Baehr, University of Utah N/A Goat polyclonal Anti-NRL, 1:500 R&D Systems CAT# AF2945; RRID: AB_2155098 Sheep polyclonal Anti-ONECUT2, 1:100 R&D Systems CAT#AF6294; RRID: AB_10640365 Rabbit polyclonal Anti-SOX9, 1:500 Millipore CAT# AB5535; RRID: AB_2239761 Rabbit polyclonal Anti-TRPM1, 1:200 Atlas Antibodies CAT# HPA014779; RRID: AB_2669090 Goat polyclonal Anti-CHAT, 1:300 Millipore CAT# AB144P; RRID: AB_2079751 Rabbit polyclonal Anti-ARR3, 1:200 Millipore CAT# AB15282; RRID: AB_1163387 Mouse monoclonal Anti-ARR3 clone 7G6, 1:200 Sigma-Aldrich CAT# MABN2636; RRID: AB_2935804 Chicken polyclonal Anti-RFP Rockland CAT# 600-901-379; RRID: AB_10704808 Lectin from Arachis hypogaea (PNA), 1:1200 Sigma-Aldrich CAT# L6135; RRID: N/A Rabbit polyclonal Anti-L/M Opsin, 1:100 Millipore CAT# AB5405; RRID: AB_177456 Goat polyclonal Anti-hIslet1, 1:200 R&D Systems CAT# AF1837; RRID: AB_2126324) Mouse monoclonal Anti-Rhodopsin, 1:400 Sigma-Aldrich CAT# R5403; RRID: AB_477464 Chicken polyclonal Anti-GFP, 1:1000 Abcam CAT# ab13970; RRID: AB_300798 Rabbit polyclonal Anti-CSNK1G1, 1:50 Abcam CAT# ab234727; RRID: N/A Sheep polyclonal Anti-CRX, 1:200 R&D Systems CAT# AF7085; RRID: AB_10993572 Rabbit polyclonal Anti-ARR3, 1:100 Atlas Antibodies CAT# HPA063129; RRID: AB_2732696 Bacterial and virus strains AAV PHP.eB ProA7-GFP Jüttner et al.10 N/A AAV PHP.eB ProA330-GFP This paper N/A AAV PHP.eB ProA330-tdTomato This paper N/A AAV PHP.eB ProA7-tdtomato-shSCR This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1A1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1E This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G3 This paper N/A AAV PHP.eB ProA7-tdtomato-shMAPK11 This paper N/A Chemicals, peptides, and recombinant proteins For detailed information about compounds used for the screening see Tables S1, S2, S3 and S5 and corresponding methods section This paper N/A Mode-of-action (MOA) compound library Canham et al.51 N/A Hoechst 33342 Thermo Fisher CAT# 62249 mTeSR1 medium STEMCELL Technologies CAT# 85850 Matrigel Matrix Corning CAT# 356230 Costar 6-well Clear TC-treated Multiple Well Plates Corning CAT# 3506 UltraPure 0.5M EDTA, pH 8.0 Invitrogen CAT# 15575020 Accutase - Enzyme Cell Detachment Medium Invitrogen CAT# 00-4555-56 (Continued on next page) ll OPEN ACCESSArticle Neuron 114, 1–20.e1–e12, July 1, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER MicroTissues 3D Petri Dish micro-mold spheroids Sigma-Aldrich CAT# Z764000 TopVision Agarose Thermo Scientific CAT# R0491 Costar 12-well Clear TC-treated Multiple Well Plates Corning CAT# 3513 DMEM/F-12, GlutaMAX supplement Gibco CAT# 31331‒028 N-2 Supplement (100X) Gibco CAT# 17502‒048 MEM Non-essential Amino Acid Solution (100X) Sigma-Aldrich CAT# M7145 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich CAT# H3149 DMEM, high glucose, GlutaMAX Supplement, pyruvate Gibco CAT# 10569‒010 Ham’s F-12 Nutrient Mix, GlutaMAX Supplement Gibco CAT# 31765‒027 B-27TM Supplement (50X), minus vitamin A Gibco CAT# 12587010 Penicillin-Streptomycin (10,000 U/mL) Gibco CAT# 15140‒122 Nunc Non-treated Flasks Thermo Scientific CAT# 159926 Anti-Adherence Rinsing Solution STEMCELL Technologies CAT# 07010 Fetal Bovine Serum Sigma-Aldrich CAT# ES-009-B Taurine Sigma-Aldrich CAT# T0625 Retinoic acid, all-trans Sigma-Aldrich CAT# R2625 Sterilin Standard 90mm Petri Dishes Thermo Scientific CAT# 101VR20 Poly(ethylene glycol) diacrylate (PEGDA) Sigma-Aldrich CAT# 455008 Porcine Gelatin Sigma-Aldrich CAT# G1890 Sucrose Sigma-Aldrich CAT# 84100 Lithium phenyl-2,4,6trimethylbenzoylphosphinate (LAP) Sigma-Aldrich CAT# 900889 Donkey Serum Sigma-Aldrich CAT# S30-M Bovine Serum Albumin Sigma-Aldrich CAT# 05482 TritonTM X-100 Sigma-Aldrich CAT# T9284 Sodium azide Sigma-Aldrich CAT# S2002 TWEEN 20 Sigma-Aldrich CAT# P9416 ProLong Gold Antifade Mountan Invitrogen CAT# P36934 Citrate Buffer, pH 6.0, 10X, Antigen Retriever Sigma-Aldrich CAT# C9999 Polystyrene CellSTACK - 5 Chamber with Vent Caps Corning CAT# 3319 PEI MAX - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences CAT# POL24765-1 DMEM, high glucose Gibco CAT# 11965-092 Millipore Stericup Quick Release Vacuum Filtration System Millipore CAT# S2GPU02RE Turbonuclease Accelagen CAT# N0103L POROS GoPure AAVX Pre-packed Column Thermo Scientific CAT# A36652 Glycine Millipore CAT# 1.04201.1000 L-Arginine Sigma-Aldrich CAT# A5006 Sodium chloride Sigma-Aldrich CAT# 31434 Trizma base Sigma-Aldrich CAT# 93350 Pluronic F-68 Thermo Scientific CAT# 24040032 (Continued on next page) ll OPEN ACCESS Article e2 Neuron 114, 1–20.e1–e12, July 1, 2026
Bioprocessing:Article Title: Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal Anti-Bassoon, 1:800 Enzo CAT# MA1-20689; RRID: AB_2066981 Mouse monoclonal Anti-ARR3, 1:500 Gift from the Laboratory of Wolfgang Baehr, University of Utah N/A Goat polyclonal Anti-NRL, 1:500 R&D Systems CAT# AF2945; RRID: AB_2155098 Sheep polyclonal Anti-ONECUT2, 1:100 R&D Systems CAT#AF6294; RRID: AB_10640365 Rabbit polyclonal Anti-SOX9, 1:500 Millipore CAT# AB5535; RRID: AB_2239761 Rabbit polyclonal Anti-TRPM1, 1:200 Atlas Antibodies CAT# HPA014779; RRID: AB_2669090 Goat polyclonal Anti-CHAT, 1:300 Millipore CAT# AB144P; RRID: AB_2079751 Rabbit polyclonal Anti-ARR3, 1:200 Millipore CAT# AB15282; RRID: AB_1163387 Mouse monoclonal Anti-ARR3 clone 7G6, 1:200 Sigma-Aldrich CAT# MABN2636; RRID: AB_2935804 Chicken polyclonal Anti-RFP Rockland CAT# 600-901-379; RRID: AB_10704808 Lectin from Arachis hypogaea (PNA), 1:1200 Sigma-Aldrich CAT# L6135; RRID: N/A Rabbit polyclonal Anti-L/M Opsin, 1:100 Millipore CAT# AB5405; RRID: AB_177456 Goat polyclonal Anti-hIslet1, 1:200 R&D Systems CAT# AF1837; RRID: AB_2126324) Mouse monoclonal Anti-Rhodopsin, 1:400 Sigma-Aldrich CAT# R5403; RRID: AB_477464 Chicken polyclonal Anti-GFP, 1:1000 Abcam CAT# ab13970; RRID: AB_300798 Rabbit polyclonal Anti-CSNK1G1, 1:50 Abcam CAT# ab234727; RRID: N/A Sheep polyclonal Anti-CRX, 1:200 R&D Systems CAT# AF7085; RRID: AB_10993572 Rabbit polyclonal Anti-ARR3, 1:100 Atlas Antibodies CAT# HPA063129; RRID: AB_2732696 Bacterial and virus strains AAV PHP.eB ProA7-GFP Jüttner et al.10 N/A AAV PHP.eB ProA330-GFP This paper N/A AAV PHP.eB ProA330-tdTomato This paper N/A AAV PHP.eB ProA7-tdtomato-shSCR This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1A1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1E This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G3 This paper N/A AAV PHP.eB ProA7-tdtomato-shMAPK11 This paper N/A Chemicals, peptides, and recombinant proteins For detailed information about compounds used for the screening see Tables S1, S2, S3 and S5 and corresponding methods section This paper N/A Mode-of-action (MOA) compound library Canham et al.51 N/A Hoechst 33342 Thermo Fisher CAT# 62249 mTeSR1 medium STEMCELL Technologies CAT# 85850 Matrigel Matrix Corning CAT# 356230 Costar 6-well Clear TC-treated Multiple Well Plates Corning CAT# 3506 UltraPure 0.5M EDTA, pH 8.0 Invitrogen CAT# 15575020 Accutase - Enzyme Cell Detachment Medium Invitrogen CAT# 00-4555-56 (Continued on next page) ll OPEN ACCESSArticle Neuron 114, 1–20.e1–e12, July 1, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER MicroTissues 3D Petri Dish micro-mold spheroids Sigma-Aldrich CAT# Z764000 TopVision Agarose Thermo Scientific CAT# R0491 Costar 12-well Clear TC-treated Multiple Well Plates Corning CAT# 3513 DMEM/F-12, GlutaMAX supplement Gibco CAT# 31331‒028 N-2 Supplement (100X) Gibco CAT# 17502‒048 MEM Non-essential Amino Acid Solution (100X) Sigma-Aldrich CAT# M7145 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich CAT# H3149 DMEM, high glucose, GlutaMAX Supplement, pyruvate Gibco CAT# 10569‒010 Ham’s F-12 Nutrient Mix, GlutaMAX Supplement Gibco CAT# 31765‒027 B-27TM Supplement (50X), minus vitamin A Gibco CAT# 12587010 Penicillin-Streptomycin (10,000 U/mL) Gibco CAT# 15140‒122 Nunc Non-treated Flasks Thermo Scientific CAT# 159926 Anti-Adherence Rinsing Solution STEMCELL Technologies CAT# 07010 Fetal Bovine Serum Sigma-Aldrich CAT# ES-009-B Taurine Sigma-Aldrich CAT# T0625 Retinoic acid, all-trans Sigma-Aldrich CAT# R2625 Sterilin Standard 90mm Petri Dishes Thermo Scientific CAT# 101VR20 Poly(ethylene glycol) diacrylate (PEGDA) Sigma-Aldrich CAT# 455008 Porcine Gelatin Sigma-Aldrich CAT# G1890 Sucrose Sigma-Aldrich CAT# 84100 Lithium phenyl-2,4,6trimethylbenzoylphosphinate (LAP) Sigma-Aldrich CAT# 900889 Donkey Serum Sigma-Aldrich CAT# S30-M Bovine Serum Albumin Sigma-Aldrich CAT# 05482 TritonTM X-100 Sigma-Aldrich CAT# T9284 Sodium azide Sigma-Aldrich CAT# S2002 TWEEN 20 Sigma-Aldrich CAT# P9416 ProLong Gold Antifade Mountan Invitrogen CAT# P36934 Citrate Buffer, pH 6.0, 10X, Antigen Retriever Sigma-Aldrich CAT# C9999 Polystyrene CellSTACK - 5 Chamber with Vent Caps Corning CAT# 3319 PEI MAX - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences CAT# POL24765-1 DMEM, high glucose Gibco CAT# 11965-092 Millipore Stericup Quick Release Vacuum Filtration System Millipore CAT# S2GPU02RE Turbonuclease Accelagen CAT# N0103L POROS GoPure AAVX Pre-packed Column Thermo Scientific CAT# A36652 Glycine Millipore CAT# 1.04201.1000 L-Arginine Sigma-Aldrich CAT# A5006 Sodium chloride Sigma-Aldrich CAT# 31434 Trizma base Sigma-Aldrich CAT# 93350 Pluronic F-68 Thermo Scientific CAT# 24040032 (Continued on next page) ll OPEN ACCESS Article e2 Neuron 114, 1–20.e1–e12, July 1, 2026
Recombinant:Article Title: Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal Anti-Bassoon, 1:800 Enzo CAT# MA1-20689; RRID: AB_2066981 Mouse monoclonal Anti-ARR3, 1:500 Gift from the Laboratory of Wolfgang Baehr, University of Utah N/A Goat polyclonal Anti-NRL, 1:500 R&D Systems CAT# AF2945; RRID: AB_2155098 Sheep polyclonal Anti-ONECUT2, 1:100 R&D Systems CAT#AF6294; RRID: AB_10640365 Rabbit polyclonal Anti-SOX9, 1:500 Millipore CAT# AB5535; RRID: AB_2239761 Rabbit polyclonal Anti-TRPM1, 1:200 Atlas Antibodies CAT# HPA014779; RRID: AB_2669090 Goat polyclonal Anti-CHAT, 1:300 Millipore CAT# AB144P; RRID: AB_2079751 Rabbit polyclonal Anti-ARR3, 1:200 Millipore CAT# AB15282; RRID: AB_1163387 Mouse monoclonal Anti-ARR3 clone 7G6, 1:200 Sigma-Aldrich CAT# MABN2636; RRID: AB_2935804 Chicken polyclonal Anti-RFP Rockland CAT# 600-901-379; RRID: AB_10704808 Lectin from Arachis hypogaea (PNA), 1:1200 Sigma-Aldrich CAT# L6135; RRID: N/A Rabbit polyclonal Anti-L/M Opsin, 1:100 Millipore CAT# AB5405; RRID: AB_177456 Goat polyclonal Anti-hIslet1, 1:200 R&D Systems CAT# AF1837; RRID: AB_2126324) Mouse monoclonal Anti-Rhodopsin, 1:400 Sigma-Aldrich CAT# R5403; RRID: AB_477464 Chicken polyclonal Anti-GFP, 1:1000 Abcam CAT# ab13970; RRID: AB_300798 Rabbit polyclonal Anti-CSNK1G1, 1:50 Abcam CAT# ab234727; RRID: N/A Sheep polyclonal Anti-CRX, 1:200 R&D Systems CAT# AF7085; RRID: AB_10993572 Rabbit polyclonal Anti-ARR3, 1:100 Atlas Antibodies CAT# HPA063129; RRID: AB_2732696 Bacterial and virus strains AAV PHP.eB ProA7-GFP Jüttner et al.10 N/A AAV PHP.eB ProA330-GFP This paper N/A AAV PHP.eB ProA330-tdTomato This paper N/A AAV PHP.eB ProA7-tdtomato-shSCR This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1A1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1E This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G3 This paper N/A AAV PHP.eB ProA7-tdtomato-shMAPK11 This paper N/A Chemicals, peptides, and recombinant proteins For detailed information about compounds used for the screening see Tables S1, S2, S3 and S5 and corresponding methods section This paper N/A Mode-of-action (MOA) compound library Canham et al.51 N/A Hoechst 33342 Thermo Fisher CAT# 62249 mTeSR1 medium STEMCELL Technologies CAT# 85850 Matrigel Matrix Corning CAT# 356230 Costar 6-well Clear TC-treated Multiple Well Plates Corning CAT# 3506 UltraPure 0.5M EDTA, pH 8.0 Invitrogen CAT# 15575020 Accutase - Enzyme Cell Detachment Medium Invitrogen CAT# 00-4555-56 (Continued on next page) ll OPEN ACCESSArticle Neuron 114, 1–20.e1–e12, July 1, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER MicroTissues 3D Petri Dish micro-mold spheroids Sigma-Aldrich CAT# Z764000 TopVision Agarose Thermo Scientific CAT# R0491 Costar 12-well Clear TC-treated Multiple Well Plates Corning CAT# 3513 DMEM/F-12, GlutaMAX supplement Gibco CAT# 31331‒028 N-2 Supplement (100X) Gibco CAT# 17502‒048 MEM Non-essential Amino Acid Solution (100X) Sigma-Aldrich CAT# M7145 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich CAT# H3149 DMEM, high glucose, GlutaMAX Supplement, pyruvate Gibco CAT# 10569‒010 Ham’s F-12 Nutrient Mix, GlutaMAX Supplement Gibco CAT# 31765‒027 B-27TM Supplement (50X), minus vitamin A Gibco CAT# 12587010 Penicillin-Streptomycin (10,000 U/mL) Gibco CAT# 15140‒122 Nunc Non-treated Flasks Thermo Scientific CAT# 159926 Anti-Adherence Rinsing Solution STEMCELL Technologies CAT# 07010 Fetal Bovine Serum Sigma-Aldrich CAT# ES-009-B Taurine Sigma-Aldrich CAT# T0625 Retinoic acid, all-trans Sigma-Aldrich CAT# R2625 Sterilin Standard 90mm Petri Dishes Thermo Scientific CAT# 101VR20 Poly(ethylene glycol) diacrylate (PEGDA) Sigma-Aldrich CAT# 455008 Porcine Gelatin Sigma-Aldrich CAT# G1890 Sucrose Sigma-Aldrich CAT# 84100 Lithium phenyl-2,4,6trimethylbenzoylphosphinate (LAP) Sigma-Aldrich CAT# 900889 Donkey Serum Sigma-Aldrich CAT# S30-M Bovine Serum Albumin Sigma-Aldrich CAT# 05482 TritonTM X-100 Sigma-Aldrich CAT# T9284 Sodium azide Sigma-Aldrich CAT# S2002 TWEEN 20 Sigma-Aldrich CAT# P9416 ProLong Gold Antifade Mountan Invitrogen CAT# P36934 Citrate Buffer, pH 6.0, 10X, Antigen Retriever Sigma-Aldrich CAT# C9999 Polystyrene CellSTACK - 5 Chamber with Vent Caps Corning CAT# 3319 PEI MAX - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences CAT# POL24765-1 DMEM, high glucose Gibco CAT# 11965-092 Millipore Stericup Quick Release Vacuum Filtration System Millipore CAT# S2GPU02RE Turbonuclease Accelagen CAT# N0103L POROS GoPure AAVX Pre-packed Column Thermo Scientific CAT# A36652 Glycine Millipore CAT# 1.04201.1000 L-Arginine Sigma-Aldrich CAT# A5006 Sodium chloride Sigma-Aldrich CAT# 31434 Trizma base Sigma-Aldrich CAT# 93350 Pluronic F-68 Thermo Scientific CAT# 24040032 (Continued on next page) ll OPEN ACCESS Article e2 Neuron 114, 1–20.e1–e12, July 1, 2026
Drug discovery:Article Title: Cell-type-focused compound screen in human organoids reveals CK1 inhibition protects cone photoreceptors from death.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal Anti-Bassoon, 1:800 Enzo CAT# MA1-20689; RRID: AB_2066981 Mouse monoclonal Anti-ARR3, 1:500 Gift from the Laboratory of Wolfgang Baehr, University of Utah N/A Goat polyclonal Anti-NRL, 1:500 R&D Systems CAT# AF2945; RRID: AB_2155098 Sheep polyclonal Anti-ONECUT2, 1:100 R&D Systems CAT#AF6294; RRID: AB_10640365 Rabbit polyclonal Anti-SOX9, 1:500 Millipore CAT# AB5535; RRID: AB_2239761 Rabbit polyclonal Anti-TRPM1, 1:200 Atlas Antibodies CAT# HPA014779; RRID: AB_2669090 Goat polyclonal Anti-CHAT, 1:300 Millipore CAT# AB144P; RRID: AB_2079751 Rabbit polyclonal Anti-ARR3, 1:200 Millipore CAT# AB15282; RRID: AB_1163387 Mouse monoclonal Anti-ARR3 clone 7G6, 1:200 Sigma-Aldrich CAT# MABN2636; RRID: AB_2935804 Chicken polyclonal Anti-RFP Rockland CAT# 600-901-379; RRID: AB_10704808 Lectin from Arachis hypogaea (PNA), 1:1200 Sigma-Aldrich CAT# L6135; RRID: N/A Rabbit polyclonal Anti-L/M Opsin, 1:100 Millipore CAT# AB5405; RRID: AB_177456 Goat polyclonal Anti-hIslet1, 1:200 R&D Systems CAT# AF1837; RRID: AB_2126324) Mouse monoclonal Anti-Rhodopsin, 1:400 Sigma-Aldrich CAT# R5403; RRID: AB_477464 Chicken polyclonal Anti-GFP, 1:1000 Abcam CAT# ab13970; RRID: AB_300798 Rabbit polyclonal Anti-CSNK1G1, 1:50 Abcam CAT# ab234727; RRID: N/A Sheep polyclonal Anti-CRX, 1:200 R&D Systems CAT# AF7085; RRID: AB_10993572 Rabbit polyclonal Anti-ARR3, 1:100 Atlas Antibodies CAT# HPA063129; RRID: AB_2732696 Bacterial and virus strains AAV PHP.eB ProA7-GFP Jüttner et al.10 N/A AAV PHP.eB ProA330-GFP This paper N/A AAV PHP.eB ProA330-tdTomato This paper N/A AAV PHP.eB ProA7-tdtomato-shSCR This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1A1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1E This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G1 This paper N/A AAV PHP.eB ProA7-tdtomato-shCSNK1G3 This paper N/A AAV PHP.eB ProA7-tdtomato-shMAPK11 This paper N/A Chemicals, peptides, and recombinant proteins For detailed information about compounds used for the screening see Tables S1, S2, S3 and S5 and corresponding methods section This paper N/A Mode-of-action (MOA) compound library Canham et al.51 N/A Hoechst 33342 Thermo Fisher CAT# 62249 mTeSR1 medium STEMCELL Technologies CAT# 85850 Matrigel Matrix Corning CAT# 356230 Costar 6-well Clear TC-treated Multiple Well Plates Corning CAT# 3506 UltraPure 0.5M EDTA, pH 8.0 Invitrogen CAT# 15575020 Accutase - Enzyme Cell Detachment Medium Invitrogen CAT# 00-4555-56 (Continued on next page) ll OPEN ACCESSArticle Neuron 114, 1–20.e1–e12, July 1, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER MicroTissues 3D Petri Dish micro-mold spheroids Sigma-Aldrich CAT# Z764000 TopVision Agarose Thermo Scientific CAT# R0491 Costar 12-well Clear TC-treated Multiple Well Plates Corning CAT# 3513 DMEM/F-12, GlutaMAX supplement Gibco CAT# 31331‒028 N-2 Supplement (100X) Gibco CAT# 17502‒048 MEM Non-essential Amino Acid Solution (100X) Sigma-Aldrich CAT# M7145 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich CAT# H3149 DMEM, high glucose, GlutaMAX Supplement, pyruvate Gibco CAT# 10569‒010 Ham’s F-12 Nutrient Mix, GlutaMAX Supplement Gibco CAT# 31765‒027 B-27TM Supplement (50X), minus vitamin A Gibco CAT# 12587010 Penicillin-Streptomycin (10,000 U/mL) Gibco CAT# 15140‒122 Nunc Non-treated Flasks Thermo Scientific CAT# 159926 Anti-Adherence Rinsing Solution STEMCELL Technologies CAT# 07010 Fetal Bovine Serum Sigma-Aldrich CAT# ES-009-B Taurine Sigma-Aldrich CAT# T0625 Retinoic acid, all-trans Sigma-Aldrich CAT# R2625 Sterilin Standard 90mm Petri Dishes Thermo Scientific CAT# 101VR20 Poly(ethylene glycol) diacrylate (PEGDA) Sigma-Aldrich CAT# 455008 Porcine Gelatin Sigma-Aldrich CAT# G1890 Sucrose Sigma-Aldrich CAT# 84100 Lithium phenyl-2,4,6trimethylbenzoylphosphinate (LAP) Sigma-Aldrich CAT# 900889 Donkey Serum Sigma-Aldrich CAT# S30-M Bovine Serum Albumin Sigma-Aldrich CAT# 05482 TritonTM X-100 Sigma-Aldrich CAT# T9284 Sodium azide Sigma-Aldrich CAT# S2002 TWEEN 20 Sigma-Aldrich CAT# P9416 ProLong Gold Antifade Mountan Invitrogen CAT# P36934 Citrate Buffer, pH 6.0, 10X, Antigen Retriever Sigma-Aldrich CAT# C9999 Polystyrene CellSTACK - 5 Chamber with Vent Caps Corning CAT# 3319 PEI MAX - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000) Polysciences CAT# POL24765-1 DMEM, high glucose Gibco CAT# 11965-092 Millipore Stericup Quick Release Vacuum Filtration System Millipore CAT# S2GPU02RE Turbonuclease Accelagen CAT# N0103L POROS GoPure AAVX Pre-packed Column Thermo Scientific CAT# A36652 Glycine Millipore CAT# 1.04201.1000 L-Arginine Sigma-Aldrich CAT# A5006 Sodium chloride Sigma-Aldrich CAT# 31434 Trizma base Sigma-Aldrich CAT# 93350 Pluronic F-68 Thermo Scientific CAT# 24040032 (Continued on next page) ll OPEN ACCESS Article e2 Neuron 114, 1–20.e1–e12, July 1, 2026
Enzyme-linked Immunosorbent Assay:
Article Title: Ferroportin-inhibitors for the use in the treatment of transfusion-dependent beta-thalassemia (TDT)
Article Snippet: .. 2 4 Serum EPO was measured using a mouse-specific DuoSet ELISA according to the manufacturer's instructions (R&D Systems, Cat. DY959). .. Plasma concentrations of Fpn127 and DFX were measured at GVK Biosciences, Hyderabad, IN.
Article Title: β -Nicotinamide mononucleotide preserves muscle strength in septic male mice
Article Snippet: .. Serum IL-6 concentrations were measured using a mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (R&D Systems, Cat. No. M6000B-1, Minneapolis, MN, USA). .. Plantarflexion force of the ankle joint was measured using a muscle strength measurement system for mice (NGM-1, Bio Research Center Co., Ltd., Nagoya, Japan).
Concentration Assay:
Suspension:
Membrane:
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 